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UNIVERSITY OF BUCHAREST
FACULTY OF PHYSICS Guest
2024-11-24 23:01 |
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Conference: Bucharest University Faculty of Physics 2024 Meeting
Section: Physics and Technology of Renewable and Alternative Energy Sources
Title:
Antioxidant effect of polyphenolic selective extracts obtained from Coriandrum sativum L. leaves.
Authors:
Cornelia NICHITA (1,2), Marcela-Elisabeta Barbinta-Patrascu (3)
Affiliation:
1) University of Bucharest, Faculty of Physics, ICUB, CTT 3Nano-SAE Research Center, MG-38, 405 Atomistilor Street, 077125, Magurele, Romania
2) National Institute for Chemical – Pharmaceutical Research and Development, 112 Vitan Avenue, 031299, Bucharest, Romania
3) Department of Electricity, Solid-State Physics and Biophysics, Faculty of Physics, University of Bucharest, 405 Atomistilor Street, P.O. Box MG-11, 077125 Magurele, Romania
E-mail
cornelia.nichita@unibuc.ro
Keywords:
Coriandrum sativum L. polyphenolic extracts, antioxidant effect, chemiluminescence
Abstract:
Coriandrum sativum L, family Apiaceae has a complex chemical composition that includes a variety of compounds such as limonene, linalool, geraniol, and borneol, which are responsible for its characteristic aroma and flavor. Additionally, Coriandrum sativum L. leaves contain flavonoids, phenolic acids, and terpenoids, which have been reported to exhibit antioxidant, anti-inflammatory, and antimicrobial properties. Amount and profile of phenolics in Coriandrum sativum L. leaves extracts depend on several factors, such as their place of origin, solvent and the methodology used for their extraction. The paper investigates the antioxidant effect of extracts obtained from Coriandrum sativum L. leaves by employing the Soxhlet extraction method, procedure followed by filtration at normal pressure and concentration the crude extract by roto-evaporation under vacuum. By UV-VIS (Jasco, Japan, V-570 spectrophotometer) spectrometric method were determined: total flavonoid contents (TFC) expressed as rutin (mg/RE g-1), by aluminum chloride colorimetric assay, caffeic acid derivatives content (CADC) expressed as 3,4-dihydroxycinnamic acid (mg/CAg-1) by using Arnows’ reagent and total polyphenolic contents (TPC) expressed as gallic acid (mg/GAE g-1) using the Folin-Ciocalteu reagent, in according to the protocol described in European Pharmacopoeia 6th edition. The antioxidant effect of Coriandrum sativum L leaves extracts was evaluated in vitro non cellular assays, by chemiluminescence method in 5-Amino-2,3-dihydrophthalazine-1,4-dione-hydrogen peroxide system, by DPPH(2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) free radical scavenging assay. All investigation methods revealed a linear correlation between the antioxidant effect and the content of phenolic compounds.
Acknowledgement:
The authors would like to thank the European Commission and CNCS/CCCDI-UEFISCDI for funding in the frame of the collaborative international consortium WaterGreenTreat financed under the 2022 Joint call of the European Partnership 101060874 — Water4All. This work was supported by a grant of the Ministry of Research, Innovation and Digitization, CNCS/CCCDI - UEFISCDI, project number COFUND-WATER4ALL-WATER Green Treat-1, No. 59/2024, within PNCDI IV and PN 23280501/2023.
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